Biochemical characteristics of a diffusible factor that induces gametophyte to sporophyte switching in the brown alga <i>Ectocarpus</i>
نویسندگان
چکیده
The haploid-diploid life cycle of the filamentous brown alga Ectocarpus involves alternation between two independent and morphologically distinct multicellular generations, sporophyte gametophyte. Deployment developmental program requires TALE homeodomain transcription factors OUROBOROS SAMSARA. In addition, generation has been shown to secrete a diffusible factor that can induce uni-spores switch from gametophyte program. Here, we determine optimal conditions for production, storage, detection this show it is heat-resistant, high molecular weight molecule. Based on combined approach involving proteomic analysis sporophyte-conditioned medium use biochemical tools characterize arabinogalactan proteins, present evidence contains AGP epitopes suggest may belong family glycoproteins.
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ژورنال
عنوان ژورنال: Journal of Phycology
سال: 2021
ISSN: ['1529-8817', '0022-3646']
DOI: https://doi.org/10.1111/jpy.13126